Scratch Testing/Wound Healing Assays have been widely optimized to study the effects of a wide range of experimental conditions. One of the most common is for chemical compound treatment and it’s effects on cell migration and proliferation.
The basic method evaluates the ability for a cell monolayer to re-grow after a manual scratch occurs, although there are now several commercial kits available to use a plug method that is more reproducible and easier to perform. The final readout for this assay is how test articles effect the cell growth rate. Many formats of these assays have been developed by TGA BioServices with many of them utilizing their own client specific controls, etc.